Fertility device ARBOR effectiveness

1. The survey conducted by Czech researchers in 1993, proved that the main characteristics of test microscope ARBOR (magnification coefficient, possibility to monitor saliva crystallization dynamics) are beyond comparison among the optical devices of personal use for female hormonal function control.
2. In 1993, during the clinical investigation of microscope ARBOR, performed by specialists of 2nd Department of Gynaecology and Obstetrics of Medical Development Institute of Kiev, 123 women of fertile age were examined for three menstrual cycles. A complete correlation between saliva / cervix mucus crystallization and colpo-cytological examination, basal temperature charting data, pupil symptom, as well as quantitative oestrogen level in blood serum was established.
3. In 1993-1994, specialists of 2nd Department of Gynaecology and Obstetrics and Department for Mother and Child Medical-Social Problems of Medical Development Institute of Kiev, examined 463 women - 180 girls between 13 to 18 years of age, 89 women with fertility disorder, 57 women with miscarriage at early pregnancy stage and 137 women hospitalised with uterus bleeding.
4. a) Overdue puberty was established in 73% of the girls. There were cases of oligo-opsomenorrhea, as well as secondary amenorrhea among them.
5. b) Infertile women testing showed the absence of ovulation being a cause of infertility. Saliva crystallization level and its growth tendency allowed picking the method of inductive ovulation treatment. Following the expedient treatment method, 67% of women of the mentioned group conceived
6. c) The examination of women with miscarriage history and/or threatened miscarriage at early pregnancy stage enabled to apply the timely treatment prior to clinical symptoms of miscarriage, what provided a positive effect.
7. d) In 92% of the women hospitalised with uterus bleeding, saliva crystallisation examination determined absolute or relative hyperestrogenia. The results complied with the respective hystological examination results, received after sampling uterine lining. Saliva crystallisation results helped to prevent and to apply more efficiently treatment of the mentioned disorder of women at fertile age

Clinical survey of test microscope ARBOR was also carried out to determine its use for natural contraception and for choosing the best time for conception based upon menstrual cycle fertile and infertile phases. It was found out, that the assessment of crystallisation patterns and dynamics helps to impeccably determine the day when estrogen level is at its peak, what shows the ovulation time and, subsequently, the end of a fertile phase for any woman with a regular menstrual cycle.

High technical characteristics of minimicroscope ARBOR and especially the possibility to keep and compare the results of saliva crystallisation dynamics enable not only to register fertile and infertile phases commencement and close as well as ovulation, but also to escape false interpretations in case of certain hormonal disorders as ovulation free period and threatened miscarriage during early stage of pregnancy.

International review (1)

In the middle of the 50, the use of woman’s saliva for optical analysis replaced the use of cervical mucus in the arborisation phenomenon studies of Andreoli, Dela Porta, Zondeka, and, particularly, the works of Spanish scientist D.Biel-Kazalsa, who established the basis of the method. The growing quantity of publications on the subject shows that the interest in this field is not fading.

Short review of arborisation phenomenon surveys made with minimicroscopes:

1. Microscope PC-2000 (Germany) testing, carried out in Spain, on the initiative of organization of Sociedas Mundial de Encuestas Medicas, where 10 gynaecologists, 40 therapeutists, 20 internists, as well as 1000 women volunteers (219 out of them due to subjective reasons discontinued the testing) participated. The survey determined:
2. a) ovulation phase during ten cycles for all the women participating;
3. b) the exception was 3 women of 37-40 years of age, who had 5 ovulation free cycles;
4. c) all the other women, who followed saliva test results and had sexual intercourses, did not get pregnant;
2. In 1992, Czech researchers (Rotta L., Mateckova E. and others) also examined the possibility to establish fertile / infertile days based on saliva crystallization monitoring with the help of PC-2000 microscope.
3. a) 58 women of different age groups from 16 to 45 years participated in the experiment. The testing was carried out for 5 months together with additional control of at least 3 classical ovulation / non-ovulation establishment methods (follicle metering, basal temperature establishment, hormonal cytology).
4. b) total 120 cycles and 1649 saliva samples were tested. Several participants had to discontinue testing due to flue epidemic (approximately 11 cycles).
5. c) The testing results show, that saliva crystallization dynamics, observed by PC-2000 microscope, are in absolute correlation with female cycle changes registered with other classical methods. The conclusion was reached, that mini-microscope PC-2000 is not only effective contraceptive device, but also helps to plan the best time for conception.
3. Comparative analysis of effectiveness of hormonal and natural contraception methods, carried out by Polish scientist E.Vuicik, showed, that effectiveness of the basal temperature establishment method and, even more, the arborisation phenomenon is more than one number higher than hormonal devices according to the universally accepted Pearl Index.

Director of Scientific-Production Centre ZAO Kiev-Donbas
Dr. of Sciences, USSR State Award Laureate
A. Moskolenko

International review (2)

Abstract
To know the days of their menstrual cycle when fecundation is possible has always been a demand felt by women and this both in the case when they want to avoid it. Many methodologies exist that enable us to verify or not the presence of a mature ovarian follicle or its happened explosion.
A very simple methodology was reached with observation and testing of the Saliva Tester; results that may be easily and directly realized by any woman, at any moment of the day, anywhere. The prospective advantages that descend as to the knowledge of the useful days for a fecundation are such that they led us to directly and correctly try this new test.

Material and Method
It has been considered the use of a mini-microscope, or “Saliva Fertility Tester”, made up of a small cylindrical body or eyepiece where we note an optical part with an opposite small slide where a sample of saliva is placed. As soon as the sample of saliva has dried, the small eyepiece body is put into a cylindrical container, that leads down to a lighting system. By focusing the lens, a saliva crystallization characteristic is pointed out, in a very near correlation with the fertility phase e.g.
     1 pic. - Infertile phase. Only chaotic crystals, in the form of sand or grains.
     2 pic. - Transition phase. Crystals are similar to small stalks, and needles.
     3 pic. - Fertile phase. "Fern branches" are observed and the crystals are grouped in thick stalks

We have taken into consideration 328 women between 15 and 50 years old, not using OCP who were placed under our observation at the out-patients' departments of the Institute of Obstetrics and Gynecology of the University “La Sapienza” in Rome. No selective principle has been adopted. We have only invited the patient to leave a sample of saliva on the slide of the mini-microscope, and afterwards age, day of the cycle in which the patient was and length of her cycle were noted. 48 women, whose sample of saliva gave the fern-like characteristically stratification were invited to undergo an ultrasonography with transvaginal sound for the control of the ovarium and of the presence or not of the pertinent follicle. 20 women accepted our request, 28 women kindly refused.

Results
Results are reported by Tables 1 and 2. In Table 1 we see, that:
    a) 48 women of 328 who, as far as anamnesis is concerned, are in the ovulation (fertile) period. 40 of these 328 women gave us the picture 3 type after test.
    b) 36 women of 328 who, as far as anamnesis is concerned, are in the transition period. 40 of these 328 women gave us the picture 2 type after test.
    c) 244 women of 328 who, as far as anamnesis is concerned, are in the infertile period. 248 of these 328 women gave us the picture 1 type after test.

Table 1. The results comparison

In Table 2 we see, that in 16 cases, equal to 80% of the total, an ovarian follicle has been stressed having dimension over 2 cm, in 4 cases a follicle having diameter less than 1 cm has been stressed.

Table 2. Ovarium echographic control

III Clinica Ostetrica e Ginecologica, Universitety degli Studi di Roma “La Sapienza” (Direttore: Prof. L. Marzeti), Professore Associato - Titolare insegnamento di Fisiopatalogia della Riproduzione
G. GALATI, E. TRAPANI, M. YCOUB, M.R. TOCCACELI, G.M. GALATI, C. FIORELLI, F. BANDIERA, A. PAOLILLO